What is a Separatome?

A separatome is defined as a sub-proteome associated with a separation technique ex. column chromatography, membrane separation, filtration, precipitation. etc. We utilize the separatome concept in conjunction with the Lotus Importance Score equation to mathematically prioritize specific modifications to the proteome via, for example, gene knockout, gene silencing, gene modification, or gene inhibition. The host cells, or sets of host cells utilizing the Lotus platform display a reduced separatome, the properties of which lead to an increase in column capacity as host cell proteins with high affinity are eliminated first.